Work Title: Microfluidic Extraction and Digital Quantification of Circulating Cell-Free DNA from Serum
Communication Type: Publication in scientific journal
Location: Sensors and Actuators B: Chemical, Vol 286 (1) : 533-539, 2019.
This work reported the developement of a microfluidic approach that combines on line a dynamic magnetic extraction procedure with droplet-based digital PCR (ddPCR). This strategy maximizes the surface area for DNA binding within the chip, in order to capture short DNA fragments, with the possibility of recovering the purified samples into picoliter volumes for high sensitivity mutation detection. The application of this technology to capture circulating cell-free DNA (ccfDNA) from serum samples of cancer patients was also demonstrated with efficiencies comparable to standard column-based DNA extraction methods.
By: Perez-Toralla, I. Pereiro, S.Garrigou, F.Di Federico, C. Proudhon, F-C. Bidard, J-L Viovy, V. Taly and S. Descroix
Stéphanie Descroix (Institut Curie, PSL Research University, CNRS, UMR 168, F-75005, Paris).